Trichomonas Medium

Trichomonas medium is based on the formula described by Kupferburg, Johnson and Sprince for the selective isolation of Trichomonas spp. (1)

The tryptone and liver extract act as carbon, nitrogen and vitamin sources in this medium. Maltose is a fermentable carbohydrate.

The agar and cysteine HCl reduce the oxygen tension in the medium which aids the growth of trichomonads.

Methylene blue is a redox indicator and allows for the visualisation of any significant oxygen diffusion in the medium.

The addition of selective agents such as chloramphenicol is recommended to inhibit bacterial species that may be present in specimens.

References
1) Kupferberg, A.B. Johnson, G., and Sprince, H. 1948. Proc. Soc. Exper. Biol. Med., 67:304-308

Reduce the oxygen tension in the medium which aids the growth of trichomonads.

Shelf Life (days):1095 days

Dehydrated Medium Appearance: Fine Powder

Dehydrated Medium Colour:  Straw

Dehydrated PH: 6.5 ±0.2

Prepared Medium Appearance: Liquid

Prepared Medium Colour: Green

prepared PH: 6.5 ± 0.2

Formula* g/L

Tryptone: 20.0

Liver extract: 12.0

Cysteine HCl: 1.6

Maltose: 1.1

Agar: 1.0

Methylene blue: 0.003

Total: 35.7

*Adjusted/supplemented as required to meet performance requirements