Selenite F Broth Base

Developed by Leifson, selenite F broth is a medium for the selective enrichment of Salmonella spp. from both clinical and food samples.

The peptone acts as a nitrogen, carbon and vitamin source. Lactose is a fermentable carbohydrate and sodium phosphate is a buffer. The medium is made selective by the addition of sodium biselenite (KM8021).

Following overnight incubation subculture(s) are usually made onto one or more of the many selective enteric solid media.

Medium for the selective enrichment of Salmonella spp

Shelf Life (days): 1095

Storage Temp (°C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Dehydrated Medium Colour: Straw

Dehydrated PH: 7.1 ± 0.2

Prepared Medium Appearance: Liquid

Prepared Medium Colour: Clear to Pale Orange

Prepared PH: 7.1 ± 0.2

Recommended Incubation: Aerobically at 35-37°C for 18-24 hours

Formula g/L

Peptone: 5.0

Lactose: 4.0

Sodium Phosphate: 10.0

Total: 19.0